Sleep and Stress Test

$249.00 $199.00


Markers testedCortisol and Melatonin (morning, noon, evening, and night)

At Home Sleep & Stress Test

Available across US except in NY, NJ, MD and RI.

The test will measure stress hormone (cortisol) and sleep hormone (melatonin)  levels using four saliva samples within 24 hours: morning, noon, evening, and night. A physician will review the results to assess whether your levels follow the natural 24 hour diurnal rhythm. You can also order a 24 cortisol test (4x) or adrenal stress test.

FREE Shipping both ways. Tested at world-class CLIA-certified labs.

Easy, Fast, Reliable and requires only few simple steps:

1. Order Online

2. Receive The Kit And Collect Sample At Home

3. Ship It Back For FREE To Our World-Class CLIA Labs

4. Receive Secure, Confidential, & Easy-To-Understand Report Within Days

Questions? Contact Us. Text: 971-205-2185 (9 am – 6 pm PST)

Test Details

What are stress and sleep hormones?

The stress hormone, cortisol, is produced in adrenal glands and has important role in metabolism to break down carbohydrates, lipids, and proteins. A well defined 24 hour circadian pattern is an important characteristic of cortisol. The levels peaks around 8 am and then gradually drop throughout the day.

The sleep hormone, melatonin, is produced by pineal gland in the brain and has an opposite trend to the cortisol cycle. It is highest in the night and drops after we wake up. Studies suggest the levels are strongly dependent on exposure to light, especially the blue light common in electronic devices.

Why measure cortisol and melatonin levels?

Change in the normal circadian rhythm can be caused by many issues including chronic stress, adrenal fatigue, burnout, or problems with adrenal glands. Chronically high levels are especially bad as they may result in increased appetite and weight gain. Excessive levels cause muscle loss, immune suppression, water retention, and bloating. Low levels are also bad since deficiency can result in fatigue, sluggishness, and low energy.

Checking cortisol levels can help identify potential problems with adrenal glands. Very high levels are indication of Cushing syndrome and unusually low levels indicate Addison’s disease. Therefore, diurnal cortisol test assessing the circadian rhythm profile is crucial to diagnose issues related to the HPA (hypothalamus, pituitary gland, and adrenal glands) hormone balance.

Any changes in melatonin cycle can results in insomnia, delay in falling asleep, and drowsiness during the day from lack of sleep. A measurement can help assess how your daily cycle compares to an expected healthy trend. Learn more about melatonin at the National Sleep Foundation.

How to test for cortisol and melatonin levels?

An easy, low cost method of measuring your levels is by using a sleep and stress test at home. Simply order the test kit, collect the saliva sample at home, ship it for free to our world class CLIA-certified labs, and find out your levels within days. Unlike a blood test, a saliva sample makes it especially useful for those fearful of needles.

We also offer 4 point cortisol test, as well as cortisol am test and cortisol pm test as an alternative. The Cortisol AM-PM Saliva Test Kit measures levels in the morning and evening.

Be informed, read more…

For a detailed discussion about symptoms of low and high cortisol levels, a comparison of cortisol blood test versus saliva test cortisol, main reasons to get tested, the convenience of an at home saliva cortisol test, the important role it plays in weight gain, adrenal fatigue, muscle loss, and more, please read: All About Cortisol. ZRT lab has an excellent discussion and plots of diurnal cortisol curves for healthy individuals as well as patients with chronic stress, chronic fatigue, and burnout symptoms. Finally, you can learn more about the intricate details of a salivary testing cortisol levels for Cushing Syndrome from the experts at Mayo Clinic.

Learn more (1): What are Adrenal Glands?

The adrenal glands are a pair of glands on top of each kidney. They produce five key hormones:

  • Three steroid hormones: testosteroneDHEA, and aldosterone
  • Two non-steroid hormones: adrenaline and noradrenaline

Adrenaline hormones have a classic 4-ring backbone structure derived from cholesterol (examples are testosterone, estrogen, progesterone).

There are two types of steroid hormones:

  • Anabolic hormones: they build muscle tissue and promote fat loss; testosterone is good example
  • Catabolic hormones: they act as a balance to anabolic hormones by accumulating fat and helping raise blood sugar by breaking down muscles and bones; cortisol is a good example

Learn more (2): What is Pineal Gland:

The pineal gland is a pine cone shaped gland that is about the size of a pea in the middle of brain. Besides the cyclic release of melatonin to control sleep cycle, it plays a key role in skin pigmentation, and release of another hormone called serotonin. This short YouTube video explains more.


Q: Can I purchase the test across US?

A: Except New York, New Jersey, Rhode Island, and Maryland our tests are available in all 46 states. State regulations in NY, NJ, RI, and MD do not allow us to ship the tests to their residents.

Q: What does the kit contain and how do I use it?

A: The kit contains four tubes for sample collection, instructions on how to collect the samples, and a form requesting basic information including date and time of collection. The directions are straight forward and easy to follow.

Q: How long does it take to receive the results?

A: You will receive the kit within 3-5 business days with a prepaid return envelope. After you ship the sample and it is received by our lab, you get the results within 5-7 business days.

Q: What do I expect from the report?

A: The report with your test results will be easy to understand and will have all the necessary details. It will mark your cortisol and melatonin levels on a healthy range for the time of the day and will specify what are the expected low and high ranges. It will briefly discuss what your numbers mean and next steps you should take to maintain healthy levels.

Q: Can I use my insurance to pay for the test?

A: You can use HSA (or FSA/MSA/HRA) accounts to pay for the tests since these are prescription tests (for further confirmation, please check the IRS publication#969). However, please ensure you are not going outside your specified max and min deductible limits. We do not have the capability to process the insurance claim. Since insurance plans vary for individuals we can not guarantee your HSA payment will always be processed by your plan. We suggest talking to your insurance provider if you have any questions.

Q: How reliable are my tests and how do I ensure my results are accurate?

A: Measuring cortisol and melatonin levels using saliva is a well researched and thoroughly documented procedure. If fact, it is reliably accurate and one of the best way to testing cortisol levels.

The variations in results are mainly from two sources: the way samples are collected and the variation from lab-to-lab. Levels are highest in the morning, when we are most energetic, and then drop during the day.

To minimize this variation, you should collect the sample within 30-60 minutes of waking up, without eating, brushing or putting anything else in your mouth that might contaminate the sample.

To minimize the variation from labs, we have partnered with CLIA-certified labs that are regulated by the states, as well as Centers for Medicare and Medicaid Services (CMS). Majority of the tests from these labs are FDA approved to ensure they meet the proper regulatory requirements. Additionally, the College of American Pathologists (CAP) requires inter-laboratory tests to ensure the ranges established by each lab do not drift or are not out of acceptable ranges. This is done by regularly testing reference samples between different labs. Finally, the labs test thousands of samples for different age groups and health conditions, and have well established reference data to compare your results against this large pool.

Q: Can you recommend what therapy should I take for cortisol levels high or low?

A: Unfortunately, no. As a lab test provider we can only test and report out data from your sample. We are not authorized to provide any medical recommendations. But we strongly encourage you to discuss the results with your doctor for next steps.

Reasons to Check

5 reasons for testing cortisol and melatonin levels:

  1. It is the first step to understand why you start to feel tired in the afternoon and evening. Our at home Sleep and Stress Test kit can help understand your energy levels throughout the day and inability to sleep in the night.
  2. Long term mental or emotional stress can raise the stress hormone levels which manifest into weight gain and adrenal fatigue. Stress, anxiety, and depression are commonly observed when levels are high. The test is an easy first step in understanding how your body is handling stress and what life style changes might be required to maintain health.
  3. If you are struggling to loose weight, a strong possibility of cortisol levels high should be assessed. If you find it difficult to fall asleep after going to bed or unable to get up early in the morning, your sleep cycle might have shifted. A Sleep and Stress Test kit is a great way to check this shift.
  4. Loss of the natural diurnal cortisol rhythm is a strong indicator of hormone imbalance and serious health issues. Drowsiness and tiredness from lack of sleep can seriously impact our ability to focus at work and may result in lower productivity.
  5. Better health results in better quality of life, and a test from not only offers insight into various health related symptoms, but it can also serve as a motivation to treat each of these symptoms and have a better quality of life!

Why with Us?

7 reasons to order from

  • No lab visits or doctor appointments
  • Saliva test of cortisol and melatonin (no blood needed)
  • World class CLIA-certified labs
  • Physician reviewed results
  • Fast, secure, and confidential reports
  • Free shipping
  • Qualifies for payment using HSA/FSA

Still wondering how do you test cortisol and melatonin levels?

  • Each at home test kit from includes a prescription and detailed review of the results by one of our physicians at no extra cost. And all the shipping is free.
  • processes your sample at the same CLIA-certified labs that doctors use. So rest assured your tests are being processed in a world class lab.
  • offers convenient, confidential, and reliable results at one of the lowest prices. And we also offer a basic morning and evening test as well as 24 hour saliva cortisol test kit, both are excellent for adrenal gland test.
  • You can order at home saliva test kit online, without requiring a doctor’s visit or prescription, thus saving time and money. You do not need to take any time off for lab visits which makes the the test very affordable.
  • Our Sleep and Stress Test kit is a salivary ELISA kit; it is more convenient than cortisol blood test or serum cortisol test as you don’t need to draw any blood using needles.

You may also like…